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pSP417
pSP417
規(guī)格:
貨期:
編號(hào):B243665
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSP417
商品貨號(hào) B243665
Designations pSP417
Depositors SM Podkovyrov
Biosafety Level 1
Vector Information
Size (kb): 9.1999998092651370
Vector: pSP417 (plasmid)
Construction: pRS415
Marker(s):ampR
Construct size (kb): 9.199999809265137
Features: marker(s): ampR
replicon: pMB1
reporter group: lacZ
MCS: EcoRI...BamHI
transcription terminator: T1
coding sequence: lacY
Applications
promoter-cloning vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--9.2; BglII--9.2; SalI--9.2; NotI--9.2; PvuI--6.2, 1.8, 0.75, 0.45.
Distributed in E. coli strain SP2 (ATCC 47089) which contains a pcnB mutation that limits pMB1-based plasmid copy number to approximately 10% of wild type. This is useful where high copy number may interfere with the fusion analysis.
A high copy number, promoter-cloning plasmid that with very low levels of background lacZ expression. Permits detection of weak transcription signals.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Simons RW, et al. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96, 1987. PubMed: 3596251

Podkovyrov SM, Larson TJ. A new vector-host system for construction of lacZ transcriptional fusions where only low-level gene expression is desirable. Gene 156: 151-152, 1995. PubMed: 7737510

Shipped freeze-dried
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