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lambda20-20
lambda20-20
規(guī)格:
貨期:
編號:B236144
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 lambda20-20
商品貨號 B236144
Designations lambda20-20
GenBank Number

X12660

Species Homo sapiens, human
Vector
Construct size (kb): 44.0
Insert
DNA: genomic
Insert lengths(kb): 14.0
Tissue: follicular lymphoma (library ATCC 77181)
Gene product: immunoglobulin switch mu, rearranged [IGHMS*]
Insert Size (kb) 14.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 15.0, 9.0; EcoRI--20.0, 15.0, 9.0; EcoRI/PvuII--17.0, 10.0, 4.6, 4.3, 3.9, 1.8, 0.6;
HindIII--40.0, 4.6; PstI--17.0, 7.0, 4.6, 2.8, 2.5, 2.0, 1.8, 1.5, 1.2, 1.15, 0.85, 0.55, 0.3; SalI--20.0, 9.0, 5.0 (triplet).
The sequenced portion also contains a PvuII, a HindIII and an EcoRI site.
This clone was isolated from an EMBL4 library, grown on E. coli LE392 and characterized at the Cytogenetics and Cancer Genetics laboratory of Memorial Sloan Kettering Cancer Center, New York. Date of isolation - Sept.-Oct. 1986.
A 2.54 kb PstI fragment from the insert has been sequenced (X12660) and contains a rearranged Smu-Cmu region.
The sequenced fragment contains 26 direct repeats 10-44 bp long, 51 inverted repeats, and more than 20 putative Ig recombinational signals.
References

Sabyasachi Ganguly, personal communication

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