国产成人综合久久久久久,亚洲国产成人久久综合碰,精品露脸国产偷人在视频,99久久精品免费看国产,国产免费一区二区三区在线观看,好吊妞国产欧美日韩免费观看,国产精品免费看久久久无码

The cells are resistant to 0.1 mM 6-thioguanine.

Unlike the parental line, these cells are sensitive to HAT.

Tested and found negative for ectromelia virus (mousepox).

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵ cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL.

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Goldsby RA, et al. Hybrid cell lines with T-cell characteristics. Nature 267: 707-708, 1977. PubMed: 301614

Hammerling GJ. T lymphocyte tissue culture lines produced by cell hybridization. Eur. J. Immunol. 7: 743-746, 1977. PubMed: 304002

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.